3^3 factorial design - Are biological replicates necessary
Hi, I am very new to DoEs and I have a quick question regarding my design and I could really do with some help. Essentially I am looking to optimise my product yield by varying 3 concentrations of DNA additions to cell culture. Ideally, I would like to run the experiment with 3 levels of variation (-1, 0, 1) and with a full factorial design which would be 27 runs and 3 centre point runs = 30 in to...