Questions regarding Response Surface Methodology (Box-Behnken design matrix)
Good morning, I am currently trying to optimize some enzymatic reactions using Response Surface Methodology, in concrete, Box-Behnken design matrix. Using the program, you choose your independent variables or factors (pH, temperature, enzyme load, etc) and assign a "low" and "high" value to each. The program then automatically calculates the "middle" value. My first question is if it is possible...