I think the problem is that your data isn't organized appropriately.
I think you're looking for a graph something like this:
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Your data is organized with your X value in the Column names and the Y values in each row. Instead you need XY pairs in rows a column to identify the "Row".
JMP makes it easy to reshape the data this way. Start by adding a new column to you table to hold the "Row" number.
Go to Cols->New Column... and complete the dialog naming the column Row, filling it with Sequence Data and adding the column before the first column.
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Next you'll want to Unhide and Unexclude all the columns that you've got hidden and excluded.
Then, go to Tables->Stack and you can stack all your 400-900 columns.
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You'll get a table with three columns and 2000 rows.
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Now you'll need to change the data type of the Label column to Numeric and the Modeling Type to Continuous.
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Now you can go to Graph Builder and drag the Label column to the X axis and the Data column to the Y axis and Row to the Overlay role.
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If you want only the range 400-500 you can rescale the axis or use a Local Data Filter on the Label column to exclude Labels greater than 500.
-Jeff