You can use Bivariate as before but include the three replicates in the regression analysis. Do not average the dilution series first. Put Dye in the By role to separate the data analysis but confine the results to one window.
Here is mock up of your study:
![Screen Shot 2020-10-26 at 12.00.02 PM.png Screen Shot 2020-10-26 at 12.00.02 PM.png](https://community.jmp.com/t5/image/serverpage/image-id/27660i7E505174ABA7C88D/image-size/large?v=v2&px=999)
Here is the resulting analysis:
![Screen Shot 2020-10-26 at 12.00.15 PM.png Screen Shot 2020-10-26 at 12.00.15 PM.png](https://community.jmp.com/t5/image/serverpage/image-id/27661iABE8045312F3AE8F/image-size/large?v=v2&px=999)
I saved the script for this analysis to the data table. I attached the data table with the mock up of your study.