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why one of the level of a factor is not included in the prediction profiler?

Hi, I am doing analysis of my bioprocess data in which I am trying to screen the effects of factors such as seeding, feeding etc on antibody productivity. For seeding I have three level 1.2, 2 and 4, however, I found that in the prediction profiler, the level 4 is missing. I am wondering why this lease square model don't take level 4 into consideration?  thanks

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