Replicate runs in DoE model

[#1]

Hi, 

I have created a custom DoE design in JMP and specified to have 3 replicate runs in the section Design Generation. However, after making the design I can only find 2 identical runs. Why so?

[#2]

If you examine your design, you should have 3 different treatment combinations that have replicates (2 identical treatment combinations for 3 treatments).  Adding replicates does not take one treatment and replicate it 3 times.

[#3]

Hi @LargeWordsPuma5,

In addition to the response from @statman, there is a slight difference in the terms used by JMP depending on the DoE platform used that explain the different results (and may create confusion):
- In a "Classical design", JMP displays "Number of Replicates", meaning here the number times to perform each run (in addition to the original runs),
- In a "Custom design" JMP displays "Number of Replicate Runs", meaning here the number of run(s) to repeat (so you'll have two identical treatments combination for the number of "replicate runs" specified).

I hope this will help clarify,

[#4]

Hi Victor, 

Thank you for your answer. 

Due to the potential biological variation in my experiment (and requirements of the journals) I want to make three technical replicates of each treatments. Is there a way to include those in the design itself using custom design? 

Thanks in advance! 

[#5]

Much depends on what questions you want to be able to answer regarding the variation (biological and other noise).  If you just run each treatment in the initial design 3 times randomly, you will be able to analyze that data in JMP and the variation captured by the replicates will be used to estimate the random errors for statistical tests (e.g., mean square error in ANOVA).

[#6]

Hi @LargeWordsPuma5,

One option could be to create your Custom Design without any replicates, and then use the platform "Augment Design" to create the replicates of your initial design : https://www.jmp.com/support/help/en/17.1/index.shtml#page/jmp/replicate-a-design.shtml#ww76833

I hope this answer will help you,