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Jan 27, 2016 6:24 PM
(545 views)

I want to know limit of detection and quantification using JMP.

How should I treat data?

2 REPLIES

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Jan 28, 2016 6:06 AM
(347 views)

In analytical chemistry, the limit of detection (LOD) is the smallest signal that is detectable with a given confidence. This usually takes the form of a sample tested against a blank or reference standard. In that case, you are talking about using a t-test with alpha equal to your confidence level. This can be done in the Fit Y by X platform and also potentially in the Matched Pairs platform depending on the specifics of your experiment.

The limit of quantification (LOQ) is more arbitrary, but the rule that I was taught is 10x the standard deviation of the blank signal. This can be determined using the distribution platform looking at the data from your reference or blank.

Getting the LOD and LOQ also depends on what form your data takes. Can you help with some details about the data (number of measurements or maybe a sample data set)? If you can the community might be able to better provide some more specific guidance.

Best,

M

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Jan 28, 2016 6:37 AM
(347 views)

To add a bit to Mike's advice above you may find this information helpful:

Estimation of a Limit of Detection