cancel
Showing results for 
Show  only  | Search instead for 
Did you mean: 
  • JMP will suspend normal business operations for our Winter Holiday beginning on Wednesday, Dec. 24, 2025, at 5:00 p.m. ET (2:00 p.m. ET for JMP Accounts Receivable).
    Regular business hours will resume at 9:00 a.m. ET on Friday, Jan. 2, 2026.
  • We’re retiring the File Exchange at the end of this year. The JMP Marketplace is now your destination for add-ins and extensions.

Discussions

Solve problems, and share tips and tricks with other JMP users.
Choose Language Hide Translation Bar
AS
AS
Level II

Batch effect adjustment using Housekeeping Genes

Hello, starting from a txt file with count data, I am analysing RNAseq data from three different independent  experiments which should be merged into a single analysis. Sample characteristics, such as disease conditions, are perfectly confounded within the batches. The use of housekeeping genes, such as those suggested by Eisenberg et al. (http://dx.doi.org/10.1016/j.tig.2013.05.010), could be one way of addressing the batch effects. Is there a way in JMP Genomics which allows specifying sets of genes which could be used to adjust the batches?

Thank you so much for your time.

1 REPLY 1

Re: Batch effect adjustment using Housekeeping Genes

Hi,

 

In JMP Genomics, you can use Loess Normalization within Expression>Normalization to specify a control set or housekeeping set of genes for normalization.  See documentation here:

http://www.jmp.com/support/downloads/JMPG90_documentation/wwhelp/wwhimpl/js/html/wwhelp.htm#href=PA_...

 

Hope this helps,

 

Chris

Chris Kirchberg, M.S.2
Data Scientist, Life Sciences - Global Technical Enablement
JMP Statistical Discovery, LLC. - Denver, CO
Tel: +1-919-531-9927 ▪ Mobile: +1-303-378-7419 ▪ E-mail: chris.kirchberg@jmp.com
www.jmp.com

Recommended Articles