I suggest that you use your SAID column as an X Grouping variable and your PCR Cycles as the X axis variable.
or ![txnelson_2-1645026998861.png txnelson_2-1645026998861.png](https://community.jmp.com/t5/image/serverpage/image-id/40060i1862B0B86137A182/image-size/medium?v=v2&px=400)
You can then right click on the graph and select "Customize" and change the Bar's Width Proportion to whatever you need it to be, for each of the levels of the X Grouping
![txnelson_1-1645026870062.png txnelson_1-1645026870062.png](https://community.jmp.com/t5/image/serverpage/image-id/40059i3E125B9A3BEB256A/image-size/medium?v=v2&px=400)
Jim