Unable to fit a 4PL curve after applying weighting (1/y^2)
I am attempting to analyze a data set that utilizes raw signal points (y, response) and concentration (x, predictor formula) for an ELISA type assay using a logistic 4P model on the data. Once I apply a 1/y^2 weighting to the raw signal points column, the graph can no longer fit giving an R = ~0.45. This same plate has been analyzed with another software okay, so I am wondering what is happening h...