From your graph, it appears you have an inconsistent number of data points within subgroup? It needs to understood whether the sampling is nested, systematic or crossed. It is always better if you can draw the sampling plan like this:

In any case, what was changing when the standard deviation was calculated? Is data 3 measures of the same foiled sample by the same operator on the same day using the same technique? If so, then the repeatability precision of the measurement equipment is what is affecting the standard deviation. While there is insufficient data to draw any conclusion, the data suggests there is evidence the repeatability of the equipment may be unstable.
"All models are wrong, some are useful" G.E.P. Box