Automation of PLA (Parallel Line Assays) analysis using JSL
Created:
Last Modified: Mar 3, 2024 8:36 AM
In the previous post ( Biological Assays using JMP - Concept and PLA (Parallel Line Assay) analysis method ) , we briefly looked at the concept of the BioAssay analysis method and the analysis method in JMP .
In this post , we would like to share how to automate (more precisely, semi-automate) the BioAssay analysis method using JMP using JSL (JMP Scripting Language) .
Using the JMP Journal function , when a data table is given, Test for Regression / Test for Parallelism / Test for Linearity ( not covered in this post ) / Relative Potency calculation and 95% confidence interval calculation were semi-automated using a script .
First , it is assumed that only two preparations (S / T) excluding Preparation U are analyzed in Case 5.1.1 Data Table of European Pharmacopoeia EP 5.3 .
The screen above is the final created JMP Journal , and analysis automation is performed in the following order .
① Data Table Preparation : In this posting , only two preparations are used . When you press the first button, you can see that the Data Table is ready as shown below .
Of course, in the case below, the data is already open , and a data table of the same format is required to apply the script in the next step .
Additionally , this data table should not be closed until the final step 5 .
② PLA (Parallel Line Assays) The first Test for Regression
When you press the “ Test for Regression ” button in the Journal, a statistical test is performed to determine whether the slope for each preparation (Preparation) is meaningful, as shown below .
In addition , since it may be difficult for non-statistics majors to interpret the above analysis results , pressing the next button “View Test Result” will print the test results (Passed / Failed) as shown below.
③ PLA (Parallel Line Assays) The second Test for Parallelism
As in ②, if you press the “ Test for Parallelism ” button in the Journal , a statistical test will be performed to determine whether the slopes of the two preparations (Preparations) are parallel, as shown below .
The “ View Test Result ” button for non-statistics majors allows test results (Passed/Failed) to be output as shown below .
④ How to find Relative Potency and 95% confidence interval
When you press the “Relative Potency & Confidence Interval” button, the result window below will be displayed.
⑤ The buttons that display all the above results on one screen are the two “Generating Report” / “Export Final Report as PDF” buttons.
When you press the first “ Generating Report ” button, the results of each of the three (Regression / Parallelism / Relative Potency) are merged horizontally for easier viewing at a glance, showing the results as shown below .
The second “ Export Final Report as PDF ” button is a script that creates a PDF file for reporting purposes . These results have all been merged vertically for ease of printing .
The final vertically merged PDF file as above is attached to this post . Please note .
This post originally written in Korean and has been translated for your convenience. When you reply, it will also be translated back to Korean.
You must be a registered user to add a comment. If you've already registered, sign in. Otherwise, register and sign in.