just looking at your figure...
It is very likely that you have Cleaved vs. something else, and for those two categories you have 6 replicate measurements for each (treated 1-6).
Your data should be formatted with at least three columns. Column 1 will have Cleaved and the other category, Column 2 will have the replicate measurements (treated 1-6), and Column 3 will have your measurement data.
Right now you have a column with lables like, "Cleaved, Treated 1". This column has two pieces of information in it that need to be split.
Use Cols>Utilities>Text to Columns, and specify a comma as the delimiter. This will give you two columns that you need.
With the data in this format, put Data in the Y role, and Column 1 in the X role. Then you will see the t-test option.
-The t-test options only show up in the red triangle menu when there are only two groups of data.
-ANOVA is an acronym for Analysis of Variance. When there is only one measurement per group, there is no variance.
I hope this helps
JMP Systems Engineer, Health and Life Sciences (Pharma)
