Hi, I am trying to analyze calcium transient data from cardiomyocytes. I moved to JMP because Prism cannot do a two way ANOVA on nested data but I cannot figure out how to run this analysis.
I have 4 experimental groups (WT SHAM, KO SHAM, WT OVX, KO OVX) across two factors (genotype (WT/KO) and surgery (SHAM/OVX)) but I don't know how to add these variables prior to running analyses. For each animal in each condition I have 6-15 cells. I haven't finished data collection so I only have 2 animals for the first two conditions (WT SH, KO SH) but I'd love to figure out how to analyze this data.
Can someone help me out?
Thank you!!
