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eberndl
Level I

Getting NonEstimable values when crossing variables due to control setup

Hi,  

 

I'm working on a dataset where we are testing the effects of 2 treatments and the delay between them, and I'm trying to figure out how to line up my variables so I can do a Dunnett's test against various controls.

 

We are giving animals a dose of drug A (Variable A), waiting some time (T), and then giving them a dose of drug B . If the animal is NOT given drug A (= 0), then T doesn't have a meaning, so I've been setting it as -1.  Similarly, there is no meaning if the animal does not receive drug B, which I've ALSO been setting at T=-1. Therefore all what I'm considering to be the controls (either no A or no B) have a T=-1

eberndl_0-1699641376236.png

 

I've been creating my graph using graph builder and then launching a standard least squares analysis.  If I use A, B and T to model the effects, I get values, but if I want to look at the interactions, everything falls apart and all my means become NonEstimable.

 

I understand that this is because there is no overlap between my T=-1 and my other time points, but I don't know how to say that a T=-1 for A=0, B=0 is the same value as a T = 0, 1 or 2.  Do I just replicate my data with multiple T's?  Do I need to put weighting on that pseudo data to make sure the standard deviations are accurate?

 

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