Yes, a. = 11. But the other questions may not be answerable because I don't know enough about the goal and structure of the study. When I think of a calibration study, I generally think of a study where one is comparing a measurement/assay system to a known or desired reference. Then usually a specific experiment is designed to answer the basic goals of the study...it looks like you are at least interested in estimating repeatability and linearity of the system. if that's the case, then the experimental design needs to support estimating those features/statistics. Generally then, one would not use the Fit Y by X platform to analyze the data but the Measurement System Analysis platforms and capabilities embedded within. Can you share more about the specific goals of your study and the structure and design of the data?