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  • Image to Table JMP extension imports single or batch images as data tables. Useful to extract curves from pictures or convert image data to curve data to use in analyses like Functional Data Explorer. Download and install the extension.

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chanson
Level I

ANOVA of Dose-Response Curve

Hello,

I have some basic questions.


I need to create a dose response curve for 3 drugs and 2 controls. How can I use 2-factor ANOVA to compare the drugs and controls? Do I make one of the drugs the dependent variable and a control and another drug as the independent variables? I'm very new to this stuff so I would appreciate some help.

Thanks!

2 REPLIES 2
Thierry_S
Super User

Re: ANOVA of Dose-Response Curve

Hi,
I think that I provided some direction relevant to this question in your other post: Setting control as baseline

Thierry R. Sornasse

Re: ANOVA of Dose-Response Curve

What do you mean by "compare?"

 

Also, you have two directions to answer the questions once you decide what comparison makes sense. The first approach is the extract a scalar value, like ED50, You won't be able to apply statistical inference, though, unless you have more than one independent curve for each sample (drug or control). Fit Curve as @Thierry_S suggested provides methods to directly compare parameter estimates or test equivalence. The second approach is to use the entire curve. The Functional Data Explorer will convert each curve into a set of 'functional principle components' that represent the salient features of the curves and can be used as a new response variable. You can them use it with the drug and control categorical variable to compare.

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