Understanding positional effects in microtiter plate-based assays is an important step in developing robust potency assays for testing biologics. Here, we present a bespoke JMP application that automates the analysis of multiple-plate experiments aimed at detecting plate bias. The application, using the raw output plate reader file, creates plate map visualisations, calculates IC50/EC50 of drug dose-response curves and analyses row and column variabilities across plates. What typically took scientists half a day of analysis, error-prone manual formatting of datasets and need of external software for curve fitting, now can be all achieved in JMP in under 1 minute.
During the talk, the microtiter map shape files were mentioned. Here the link: Microtiter Well Plate Map Shape Definition File - 6, 12, 24, 48, 96, 384, 1536 and 9600 well
