Hi I want to compare between two treatments: 1) No fertilizer and 2) Fertilizer. I wanted to know whether there is an increase in yield due to the fertilizer application. Two parallel blocks were selected, in the first block no fertilizer was applied whereas in the second block the fertilizer was applied. Fifteen samples were harvested from each block. I would like to use the paired t test and there are different methods in JMP. I thought to use the distribution and then test means. My question is that is adding the column “n” for “frequency” is correct? Please explain why or why not. If I use n as frequency in the data analysis the results changes as the degree of freedom increase from 14 to 29. 
