Running an ELISA test I got the best fit for the standard curve using fit special and log/log transformation as a quadratic function: Ln(OD) = -4.139548 + 1.301394*Ln(Concentration of α-CD3 [ng/ml]) - 0.0934906*Ln(Concentration of α-CD3 [ng/ml])^2. Is the square operator applicable for concentration only or for its logarithmic derivative. How to convert this equation to be able to calculate concentration from the measured OD? I prefer an interactive explanation. Thanks.
Bivariate(
Y( :OD ),
X( :Name( "Concentration of α-CD3 [ng/ml]" ) ),
Fit Special(
xTran( "Log" ),
yTran( "Log" ),
Degree( 2 ),
{Line Color( {142, 176, 40} )}
),
SendToReport(
Dispatch(
{},
"1",
ScaleBox,
{Scale( "Log" ), Min( 5 ), Max( 400 ), Inc( 1 ), Minor Ticks( 1 )}
),
Dispatch(
{},
"2",
ScaleBox,
{Scale( "Log" ), Min( 0.1 ), Max( 2 ), Inc( 1 ), Minor Ticks( 1 )}
)
)
)
