Dear JMP Community,
My question is about a DOE performed on ELISA method.
The response is the Absorbance and there are 3 factors which are dilution of different reagents (standard, parameter B and parameter C) - Refer to graph attached to visualize the type of data I have.
I’m looking for optimizing the three factors to get a plateau equal to 2 for absorbance. But I would like also to be at “the beginning” of the plateau.
I managed to optimize to get a plateau around 2 (I used 4PL modeling). But I don’t know how to be at the beginning of the plateau. Would you have ideas to share to parameter this need?
I updated the document attached with a new schema to detail what I mean by "beginning of the plateau".
It could correspond to an IC99 for example.
Is it clearer for you?
I'll try to be clearer :
- The response studied is the absorbance.
It is modeled by a 4PL curve when represented in function of the dilution of the standard used in the analysis: Absorbance = f (Log 1/[standard])
- I'm analysing three factors:
- Dilution of the standard (x-axis)
- Parameter B (dilution of a reagent used in the analysis)
- Parameter C (dilution of a second reagent used in the analysis)
As we can observe on the first schema, Parameters B and C will mostly affect the value of the plateau (or also difference min-max of the curves).
The ideal configuration I'm looking for is the point that check 2 conditions
- Absorbance plateau = 2
- Dilution of the standard corresponding to the beginning of plateau (IC99 maybe)
At the end, I would like to define value of the three factors that allows me to reach the ideal configuration.
My issue is that I don't know how to configure this ideal configuration in the profiler/desirability to get this optimization.