Use of Definitive Screening Designs for Optimising Immunoassay
Emmanuel Romeu, R&D Project Manager, Beckman Coulter (Danaher Corporation)Magali Karagueuzian, Development Specialist, Beckman Coulter (Danaher Corporation)Developing an assay for diagnosing human antibody is particularly difficult while using poorly soluble protein. We optimised the coupling process of the protein on the solid phase (concentration, pH, glycerol) and the measurement conditions of ...