I have a set of data that seemed straight forward, but now I am sure how to proceed. The experiment consisted of a single factor (fertilizer) with four treatments (fertilizer levels) and eight replicates (plants) of each treatment. Each replicate then had 5 data points collected on it (ie, 5 leaves from each plant were measured for area at a single time point). I am not sure what the proper way to handle this data is. Do I consider this a repeated measures, since each replicate had multiple measurements? Can I consider the repeat observations as subplots and run a split-plot analysis? Or can I just average the data points for each replicate, and run an ANOVA?
... View more