Speeding up assay development with automated workflow for assessing plate bias of microtiter plate assays.

tomwit — Mon, 10 Oct 2022 17:01:55 GMT

Understanding positional effects in microtiter plate-based assays is an important step in developing robust potency assays for testing biologics. Here, we present a bespoke JMP application that automates the analysis of multiple-plate experiments aimed at detecting plate bias. The application, using the raw output plate reader file, creates plate map visualisations, calculates IC50/EC50 of drug dose-response curves and analyses row and column variabilities across plates. What typically took scientists half a day of analysis, error-prone manual formatting of datasets and need of external software for curve fitting, now can be all achieved in JMP in under 1 minute.

During the talk, the microtiter map shape files were mentioned. Here the link:

topic Speeding up assay development with automated workflow for assessing plate bias of microtiter plate assays. in United Kingdom JMP Users Group Discussions

Speeding up assay development with automated workflow for assessing plate bias of microtiter plate assays.