https://community.jmp.com/t5/Discussions/How-to-constrain-asymptotes-in-4PL-fitting/m-p/458318#M70343 A little background info:<BR />New user of JMP here, running JMP16 and know how to perform basic 4PL fitting and analysis with JMP.<BR /><BR />Here is the question:<BR />We often need to set values of upper or lower asymptote when fitting incomplete IC50 curves with 4PL analysis.<BR />Is there a way to do this with JMP?<BR /><BR />Thanks Fri, 09 Jun 2023 14:42:24 GMT GroupSquareWolf 2023-06-09T14:42:24Z https://community.jmp.com/t5/Discussions/How-to-constrain-asymptotes-in-4PL-fitting/m-p/458318#M70343 A little background info:<BR />New user of JMP here, running JMP16 and know how to perform basic 4PL fitting and analysis with JMP.<BR /><BR />Here is the question:<BR />We often need to set values of upper or lower asymptote when fitting incomplete IC50 curves with 4PL analysis.<BR />Is there a way to do this with JMP?<BR /><BR />Thanks Fri, 09 Jun 2023 14:42:24 GMT https://community.jmp.com/t5/Discussions/How-to-constrain-asymptotes-in-4PL-fitting/m-p/458318#M70343 GroupSquareWolf 2023-06-09T14:42:24Z https://community.jmp.com/t5/Discussions/How-to-constrain-asymptotes-in-4PL-fitting/m-p/458477#M70347 <P>You will need to use a custom model. it will be the same model as in the Model Library of the Nonlinear platform or one of the 4PLC models in Fit Curve, but instead of 4 parameters, you will only use 3 of them. The 4th is the asymptote, and set this in the formula to the constant that you want to use.</P> <P>&nbsp;</P> <P>Please see the JMP Help about using the <A href="https://www.jmp.com/support/help/en/16.2/#page/jmp/create-a-formula-using-the-model-library.shtml#" target="_self">Model Library</A>. Create the 4PLC model as a column library, then edit the formula and change one of the two asymptote parameters to the desired constant.</P> Fri, 04 Feb 2022 12:38:49 GMT https://community.jmp.com/t5/Discussions/How-to-constrain-asymptotes-in-4PL-fitting/m-p/458477#M70347 Mark_Bailey 2022-02-04T12:38:49Z