topic Re: How to compare paired data sets include the variation on the samples in Discussions

How to compare paired data sets include the variation on the samples

ANLUN — Fri, 09 Jun 2023 00:17:18 GMT

Feeling i meeting the wall with comparing these two methods I'm testing.

I have 2 methods (1 and 2) both analysed on the same sample. For both methods triplicates have been measured for each sample. So now I want to compare method 1 vs 2 including the know variation to see if statistically significant?

Re: How to compare paired data sets include the variation on the samples

Mark_Bailey — Tue, 16 Jun 2020 14:54:49 GMT

What is this study, an analytical method comparison? An equivalence study for instrument replacement? Is one of the methods a reference? What do you mean by, "So now I want to compare method 1 vs 2 including the know variation to see if statistically significant?"

Your data is pre-summarized, so you you could use Bivariate platform with the mean and then select Fit Orthogonal to compare the null hypothesis of an intercept = 0 and a slope = 1. You could use Matched Pairs platform if you are looking for a difference in the mean.

I would recommend that you include the individual replicate measurements instead of summarizing the data into the mean and standard deviation. You could use a Fit Least Squares after stacking the measurement columns. The measurement in the Y role and then enter Sample as a random effect.

Hard to say much more given we know practically nothing about your study, your intentions, or your needs.

Re: How to compare paired data sets include the variation on the samples

ron_horne — Wed, 17 Jun 2020 12:28:06 GMT

Hi @ANLUN

if you are looking for inspiration from the variety of options for method comparison try this add in:

Otherwise, we would need to know much more before being able to help.