topic Re: interpolate x values from a standard curve in Discussions https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13686#M12879 <HTML><HEAD></HEAD><BODY><P>Use the "nonlinear" platform under "analyze" and "modeling". </P><P></P><P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="9410_pastedImage_0.png" style="width: 465px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/1947i9C94779ABFB4D475/image-size/medium?v=v2&amp;px=400" role="button" title="9410_pastedImage_0.png" alt="9410_pastedImage_0.png" /></span></P><P></P><P>After fitting curves, you can use the "Custom Inverse Prediction" for interpolating "x" values.</P><P></P><P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="9411_pastedImage_1.png" style="width: 474px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/1948i30CCBD2525726DC1/image-size/medium?v=v2&amp;px=400" role="button" title="9411_pastedImage_1.png" alt="9411_pastedImage_1.png" /></span></P></BODY></HTML> Wed, 19 Oct 2016 00:09:30 GMT bwanders 2016-10-19T00:09:30Z interpolate x values from a standard curve https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13685#M12878 <HTML><HEAD></HEAD><BODY><P>Hello,</P><P></P><P>We have five control samples with known concentration and a quantitative assay result. Using the controls, we can draw a standard curve so that the concentration is represented on the x-axis and the quantitative assay result is on the y axis. The data is expected to be linear. I have clinical samples with quantitative assay result, but unknown concentration.&nbsp; I would like to determine the concentration of the clinical samples based on the quantitative assay result. </P><P></P><P>Using the fit Y by X function, I fit a line and obtain the regression line equation. I can solve the equation for x and set a formula in jmp to solve for x. </P><P>The challenge is that for each clinical run I will have a standard curve for each fluorophore, and a new standard curve for each new clinical run. Therefore, I will have different equations and will need to write different formula to solve for x.</P><P>Is there a more automated way for me to solve for x? I have used GraphPad prism in the past (describe in this link: <A href="http://www.graphpad.com/support/faqid/1753/" style="font-size: 10pt; line-height: 1.5em;" title="http://www.graphpad.com/support/faqid/1753/">GraphPad - FAQ 1753 - Prism 3 -- Calculating "Unknown" Concentrations using a Standard Curve)</A><SPAN style="font-size: 10pt; line-height: 1.5em;">and I would imagine that there is a more straightforward way for me to interpolate my x values with JMP. I would really appreciate a step by step procedure as I am a relatively new JMP user. </SPAN><SPAN style="font-size: 10pt; line-height: 1.5em;">Thank you!</SPAN></P></BODY></HTML> Wed, 05 Aug 2015 04:09:26 GMT https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13685#M12878 medusorca 2015-08-05T04:09:26Z Re: interpolate x values from a standard curve https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13686#M12879 <HTML><HEAD></HEAD><BODY><P>Use the "nonlinear" platform under "analyze" and "modeling". </P><P></P><P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="9410_pastedImage_0.png" style="width: 465px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/1947i9C94779ABFB4D475/image-size/medium?v=v2&amp;px=400" role="button" title="9410_pastedImage_0.png" alt="9410_pastedImage_0.png" /></span></P><P></P><P>After fitting curves, you can use the "Custom Inverse Prediction" for interpolating "x" values.</P><P></P><P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="9411_pastedImage_1.png" style="width: 474px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/1948i30CCBD2525726DC1/image-size/medium?v=v2&amp;px=400" role="button" title="9411_pastedImage_1.png" alt="9411_pastedImage_1.png" /></span></P></BODY></HTML> Wed, 19 Oct 2016 00:09:30 GMT https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13686#M12879 bwanders 2016-10-19T00:09:30Z Re: interpolate x values from a standard curve https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13687#M12880 <HTML><HEAD></HEAD><BODY><P>Hello,</P><P></P><P>Inverse prediction is available is several platforms including fit model.</P><P></P><P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="9425_2015-08-05_16-30-43.png" style="width: 655px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/1961iE7EDF837A2D537CA/image-size/medium?v=v2&amp;px=400" role="button" title="9425_2015-08-05_16-30-43.png" alt="9425_2015-08-05_16-30-43.png" /></span></P><P></P><P>Best,</P><P>Bill</P></BODY></HTML> Wed, 19 Oct 2016 00:11:07 GMT https://community.jmp.com/t5/Discussions/interpolate-x-values-from-a-standard-curve/m-p/13687#M12880 Bill_Worley 2016-10-19T00:11:07Z