https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898246#M105816 <P>You need to use the Matched Pairs platform</P> <P>&nbsp; &nbsp; &nbsp;Analyze=&gt;Specialized Modeling=&gt;Matched Pairs</P> <P>Use your Morining and Afternoon columns as your Y, Paired Response</P> <P>Use your Subject Time column as the By variable</P> <P>&nbsp;</P> <P>Go to</P> <P>&nbsp; &nbsp; &nbsp;Help=&gt;JMP Online Help=&gt;Matched Pairs</P> <P>To get documentation and examples</P> Tue, 02 Sep 2025 00:10:28 GMT txnelson 2025-09-02T00:10:28Z https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898228#M105812 <P>Hello there,</P> <P>I am doing a project where I need to compare morning and afternoon</P> <P>protein concentrations for some 600 proteins in samples from 5 individuals. I am using JMP Pro 18 Student Edition to do this. Lately, I am attempting to perform a paired T-test for all the proteins' concentrations in the morning and afternoon for each subject (1-5). I am not quite sure how to organize the data to get the software to do what I want it to do, and am looking for some guidance. Any help would be appreciated, this is my first time using JMP and doing a comparison like this. Attached I have an example of what the data looks like. I am open to all suggestions, and thank you for taking the time to read this!</P> Mon, 01 Sep 2025 20:19:49 GMT https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898228#M105812 CompositeLemur6 2025-09-01T20:19:49Z https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898242#M105813 <P>Here is the structure of the data that I suggest.&nbsp; It will allow you to take advantage of using row exclusion and By group processing to get your Paired t-Tests using the Fit Y by X platform</P> <P><span class="lia-inline-image-display-wrapper lia-image-align-inline" image-alt="txnelson_0-1756759763323.png" style="width: 677px;"><img src="https://community.jmp.com/t5/image/serverpage/image-id/81731i49D287FEC64E91DD/image-dimensions/677x446?v=v2" width="677" height="446" role="button" title="txnelson_0-1756759763323.png" alt="txnelson_0-1756759763323.png" /></span></P> <P>&nbsp;</P> Mon, 01 Sep 2025 20:49:50 GMT https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898242#M105813 txnelson 2025-09-01T20:49:50Z https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898243#M105814 <P>Thank you so much for the suggestion! How might I actually perform this paired t-test, stepwise, if you would be so kind as to help me?&nbsp; I am not quite sure where the variables would be assigned. No worries if you cannot, and thank you again.</P> Mon, 01 Sep 2025 21:36:03 GMT https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898243#M105814 CompositeLemur6 2025-09-01T21:36:03Z https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898246#M105816 <P>You need to use the Matched Pairs platform</P> <P>&nbsp; &nbsp; &nbsp;Analyze=&gt;Specialized Modeling=&gt;Matched Pairs</P> <P>Use your Morining and Afternoon columns as your Y, Paired Response</P> <P>Use your Subject Time column as the By variable</P> <P>&nbsp;</P> <P>Go to</P> <P>&nbsp; &nbsp; &nbsp;Help=&gt;JMP Online Help=&gt;Matched Pairs</P> <P>To get documentation and examples</P> Tue, 02 Sep 2025 00:10:28 GMT https://community.jmp.com/t5/Discussions/How-to-do-Paired-T-Test-with-Before-After-Data-and-Multiple/m-p/898246#M105816 txnelson 2025-09-02T00:10:28Z